6-Phosphogluconic Dehydrogenase from yeast, lyophilized powder, 3.0-6.0 units/mg solid

Code: P4553-25UN D2-231

Application

6-Phosphogluconic Dehydrogenase from yeast has been used in hexokinase (HK) and xylose reductase (XR) activities in cell lysate.

Biochem/physiol Act...


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Your Price
€162.60 EACH
€200.00 inc. VAT

Application

6-Phosphogluconic Dehydrogenase from yeast has been used in hexokinase (HK) and xylose reductase (XR) activities in cell lysate.

Biochem/physiol Actions

Enzyme activity is determined by measuring the reduction of NAD+ or NADP+. High levels of NADPH are believed to inhibit the enzyme, while 6-phosphogluconate acts to activate the enzyme.

General description

6-Phosphogluconic Dehydrogenase from yeast comprises of a NADP+ binding domain, α helical hydrophobic interaction domain and a C-terminal domain. It exists as dimer and the C-terminal region is crucial for the substrate binding and release.

Packaging

25 units in glass bottle

Unit Definition

One unit will oxidize 1.0 µmole of 6-phospho-D-gluconate to D-ribulose 5-phosphate and CO2 per min at pH 7.4 at 37 °C in the presence of NADP+.

biological sourceyeast
formlyophilized powder
mol wt52 kDa
Quality Level200
specific activity≥3.0 units/mg solid
storage temp.2-8°C
Cas Number9073-95-4
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